il 3 (R&D Systems)
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94
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R&D Systems
il 3
Il 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+3/Recombinant+Mouse+IL-3+Protein/bio_rxiv__64898__2025__12__22__696045-141-32-33
Average 94 stars, based on 171 article reviews
Il 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+3/Recombinant+Mouse+IL-3+Protein/bio_rxiv__64898__2025__12__22__696045-141-32-33
Average 94 stars, based on 171 article reviews
il 3 - by Bioz Stars,
2026-09
94/100 stars
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Recombinant:Article Title: STX-721, a Covalent EGFR/HER2 Exon 20 Inhibitor, Utilizes Exon 20–Mutant Dynamic Protein States and Achieves Unique Mutant Selectivity Across Human Cancer Models Article Snippet: Ba/F3 cells (RRID: CVCL_0161) were purchased from Cobioer, and experiments were performed at Pharmaron. .. Ba/F3 cells were grown in RPMI media supplemented with 10% FBS (Thermo Fisher Scientific) and 10 ng/mL Article Title: Mast cells promote choroidal neovascularization in a model of age-related macular degeneration Article Snippet: .. Bone marrow cells were flushed from the femurs and tibias using RPMI 1640, centrifuged at 1200 rpm for 5 min at room temperature, and resuspended in RPMI 1640 supplemented with 2 mM L-glutamine, 0.07% β-mercaptoethanol, 10 mM HEPES, 10% fetal calf serum (Wisent Bioproducts #085–150), 1% antibiotics (penicillin and streptomycin), and 30 ng/mL of Article Title: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential Article Snippet: BM cells were harvested by flushing femurs and tibias of 4–8 week old female mice with 10 ml RP10 (RPMI 1640 (Invitrogen), supplemented with 10 % (v/v) FBS, 50 mM 2–mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), pelleted by centrifugation, resuspended in 4 ml RP10, loaded on 3 ml Ficoll-Paque (GE Healthcare) and separated by centrifugation at 450 g for 30 min. .. The entire supernatant was collected (discarding only 500 μl including the cell pellet), diluted with 45 ml of PBS containing 1% FBS, pelleted at 800 g for 10 min, followed by resuspension in 10 ml RP10, centrifugation at 450 g for 5 min and resuspension at a concentration of 5 × 10 5 cells/ml in RP10 containing Article Title: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential. Article Snippet: Generation and cell culture of Hoxa7-TPO progenitor cell lines BM cells were harvested by flushing femurs and tibias of 4–8 week old female mice with 10ml RP10 (RPMI 1640 (Invitrogen), supplemented with 10 % (v/v) FBS, 50mM 2–mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), pelleted by centrifugation, resuspended in 4ml RP10, loaded on 3ml Ficoll-Paque (GE Healthcare) and separated by centrifugation at 450 g for 30min. .. The entire supernatant was collected (discarding only 500 μl including the cell pellet), diluted with 45ml of PBS containing 1% FBS, pelleted at 800 g for 10min, followed by resuspension in 10ml RP10, centrifugation at 450 g for 5min and resuspension at a concentration of 5 × 105 cells/ml in RP10 containing Article Title: Inhibiting the alarmin‐driven hematopoiesis‐stromal cell crosstalk in primary myelofibrosis ameliorates bone marrow fibrosis Article Snippet: .. Isolated BM cells were resuspended at a concentration of 5 × 105 cells/mL in Iscove's modified Dulbecco's medium (IMDM) (GIBCO) with Article Title: STX-721, a Covalent EGFR/HER2 Exon 20 Inhibitor, Utilizes Exon 20–Mutant Dynamic Protein States and Achieves Unique Mutant Selectivity Across Human Cancer Models Article Snippet: Ba/F3 cells (RRID: CVCL_0161) were purchased from Cobioer, and experiments were performed at Pharmaron. .. Ba/F3 cells were grown in RPMI media supplemented with 10% FBS (Thermo Fisher Scientific) and 10 ng/mL Article Title: Loss of lipid asymmetry facilitates plasma membrane blebbing by decreasing membrane lipid packing. Article Snippet: .. BaF3 cell lines (kind gift of Shigekazu Nagata’s lab, Osaka University) (8) were cultured in RPMI 1640 medium supplemented with 50 μM β- mercaptoethanol and 45 U/mL Article Title: Unique compound with anti-allergic action: inhibition of Lyn kinase activity by KIRA6 Article Snippet: Anti-IgE antibodies were purchased from Bethyl Laboratories (Montgomery, TX, United States). .. Centrifugation:Article Title: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential Article Snippet: BM cells were harvested by flushing femurs and tibias of 4–8 week old female mice with 10 ml RP10 (RPMI 1640 (Invitrogen), supplemented with 10 % (v/v) FBS, 50 mM 2–mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), pelleted by centrifugation, resuspended in 4 ml RP10, loaded on 3 ml Ficoll-Paque (GE Healthcare) and separated by centrifugation at 450 g for 30 min. .. The entire supernatant was collected (discarding only 500 μl including the cell pellet), diluted with 45 ml of PBS containing 1% FBS, pelleted at 800 g for 10 min, followed by resuspension in 10 ml RP10, centrifugation at 450 g for 5 min and resuspension at a concentration of 5 × 10 5 cells/ml in RP10 containing Article Title: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential. Article Snippet: Generation and cell culture of Hoxa7-TPO progenitor cell lines BM cells were harvested by flushing femurs and tibias of 4–8 week old female mice with 10ml RP10 (RPMI 1640 (Invitrogen), supplemented with 10 % (v/v) FBS, 50mM 2–mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), pelleted by centrifugation, resuspended in 4ml RP10, loaded on 3ml Ficoll-Paque (GE Healthcare) and separated by centrifugation at 450 g for 30min. .. The entire supernatant was collected (discarding only 500 μl including the cell pellet), diluted with 45ml of PBS containing 1% FBS, pelleted at 800 g for 10min, followed by resuspension in 10ml RP10, centrifugation at 450 g for 5min and resuspension at a concentration of 5 × 105 cells/ml in RP10 containing Concentration Assay:Article Title: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential Article Snippet: BM cells were harvested by flushing femurs and tibias of 4–8 week old female mice with 10 ml RP10 (RPMI 1640 (Invitrogen), supplemented with 10 % (v/v) FBS, 50 mM 2–mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), pelleted by centrifugation, resuspended in 4 ml RP10, loaded on 3 ml Ficoll-Paque (GE Healthcare) and separated by centrifugation at 450 g for 30 min. .. The entire supernatant was collected (discarding only 500 μl including the cell pellet), diluted with 45 ml of PBS containing 1% FBS, pelleted at 800 g for 10 min, followed by resuspension in 10 ml RP10, centrifugation at 450 g for 5 min and resuspension at a concentration of 5 × 10 5 cells/ml in RP10 containing Article Title: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential. Article Snippet: Generation and cell culture of Hoxa7-TPO progenitor cell lines BM cells were harvested by flushing femurs and tibias of 4–8 week old female mice with 10ml RP10 (RPMI 1640 (Invitrogen), supplemented with 10 % (v/v) FBS, 50mM 2–mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), pelleted by centrifugation, resuspended in 4ml RP10, loaded on 3ml Ficoll-Paque (GE Healthcare) and separated by centrifugation at 450 g for 30min. .. The entire supernatant was collected (discarding only 500 μl including the cell pellet), diluted with 45ml of PBS containing 1% FBS, pelleted at 800 g for 10min, followed by resuspension in 10ml RP10, centrifugation at 450 g for 5min and resuspension at a concentration of 5 × 105 cells/ml in RP10 containing Article Title: Inhibiting the alarmin‐driven hematopoiesis‐stromal cell crosstalk in primary myelofibrosis ameliorates bone marrow fibrosis Article Snippet: .. Isolated BM cells were resuspended at a concentration of 5 × 105 cells/mL in Iscove's modified Dulbecco's medium (IMDM) (GIBCO) with Isolation:Article Title: Inhibiting the alarmin‐driven hematopoiesis‐stromal cell crosstalk in primary myelofibrosis ameliorates bone marrow fibrosis Article Snippet: .. Isolated BM cells were resuspended at a concentration of 5 × 105 cells/mL in Iscove's modified Dulbecco's medium (IMDM) (GIBCO) with Modification:Article Title: Inhibiting the alarmin‐driven hematopoiesis‐stromal cell crosstalk in primary myelofibrosis ameliorates bone marrow fibrosis Article Snippet: .. Isolated BM cells were resuspended at a concentration of 5 × 105 cells/mL in Iscove's modified Dulbecco's medium (IMDM) (GIBCO) with Cell Culture:Article Title: Loss of lipid asymmetry facilitates plasma membrane blebbing by decreasing membrane lipid packing. Article Snippet: .. BaF3 cell lines (kind gift of Shigekazu Nagata’s lab, Osaka University) (8) were cultured in RPMI 1640 medium supplemented with 50 μM β- mercaptoethanol and 45 U/mL |